本課程主要講授發表在Nature Methods期刊中的新興醫學及生命科學研究方法，並探討這些技術的未來應用。
課程簡述
Brief course description
本課程主要講授發表在Nature Methods期刊中的新興醫學及生命科學研究方法，並探討這些技術的未來應用。
This lecture will be given in English.
The lectures will focus on introducing select key methodologies published in the prestigious journal Nature Methods, as well as discussing their potential future implications. The teachers will introduce 17 different method topics and the lecture will be given in a hybrid mode (physical ＋ online).
This lecture will be given in English.
The lectures will focus on introducing select key methodologies published in the prestigious journal Nature Methods, as well as discussing their potential future implications. The teachers will introduce 17 different method topics and the lecture will be given in a hybrid mode (physical ＋ online).
課程說明
Course Description
Nature Methods is a monthly journal publishing novel methods and significant improvements to basic life sciences research techniques. The journal is aimed at a broad, interdisciplinary audience of academic and industry researchers actively involved in laboratory practice. It provides researchers with new tools to conduct their research and places a strong emphasis on immediate practical relevance and potential to advance new biological applications.
We noted that each paper published in Nature Methods must be accompanied by strong validation, an application to an important biological question and results illustrating its performance in comparison to available approaches. Thus, papers are selected for publication based on broad interest, thorough assessments of methodological performance and comprehensive technical descriptions that facilitate immediate application. Accordingly, we believe our students may benefit a lot by learning these new methods.
We noted that each paper published in Nature Methods must be accompanied by strong validation, an application to an important biological question and results illustrating its performance in comparison to available approaches. Thus, papers are selected for publication based on broad interest, thorough assessments of methodological performance and comprehensive technical descriptions that facilitate immediate application. Accordingly, we believe our students may benefit a lot by learning these new methods.
指定用書
Textbook Book
| ♠ | Nature methods |
| The electronic international standard serial number (EISSN) is 15487105. | |
| https://www.nature.com/nmeth/ | |
| https://www.nature.com/nmeth/articles?type=method-to-watch |
教學方式
Teaching Method
Each lecture will consist of two parts. The first part will feature lectures given by individual teachers, while the second part will involve student presentations and discussions guided by the teachers
教學進度
Course Content
Course Content
Please see the following table.
| Week | Date | Teacher | Topic | |
| 1 | 09/14 | Lily Wang | General lecture introduction | |
| Topic-1: Method to watch 2022: Imaging without the labels https://www.nature.com/articles/s41592-02101376-0 Topic-2: Organic dyes for live imaging https://www.nature.com/articles/s41592-020-01032-z | ||||
| 2 | 09/21 | Wei-Ching Chen | Topic-3: Method to watch 2020: Tools for metabolomics http:://www.nature.com/articles/s41592-019-0710-6 | |
| Topic-4: Method to watch 2023: Annotating unknown metabolites http://www.nature.com/articles/s41592-022-01735-5 | ||||
| 3 | 09/28 | Teacher’s | day | |
| 4 | 10/05 | Pei-Chun Lin | Topic-5: ClampFISH Nat Methods 19, 1403–1410 (2022). https://doi.org/10.1038/s41592-022-01653-6 | |
| Student presentation | ||||
| 5 | 10/12 | Ya-Ching Chou | Topic-6: Joint profiling of DNA methylation and chromatin architecture in single cells https://www.nature.com/articles/s41592-0190502-z | |
| Student presentation | ||||
| 6 | 10/19 | I-Ju Lee | Topic-7: Next-Generation Expansion Microscopy https://www.nature.com/articles/s41592-023-01793-3 and related articles | |
| Student presentation | ||||
| 7 | 10/26 | Yu-Ting Lin | Topic-8: Controlling cellular activities with light Nat Methods 20, 357–358 (2023) https://www.nature.com/articles/s41592-022-01745-3 | |
| Student presentation | ||||
| 8 | 11/02 | Pei-Chun Lin | Topic-9: Cryo-electron tomography Nat Methods (2023). https://doi.org/10.1038/s41592023-01783-5 | |
| Student presentation | ||||
| 9 | 11/09 | I-Ju Lee | Topic-10: Methods to watch 2023: A light switch for targeted genomics https://www.nature.com/articles/s41592-022-01733-7 | |
| Student presentation | ||||
| 10 | 11/16 | Ya-Ching Chou | Topic-11: SpaGCN: Integrating gene expression, spatial location and histology to identify spatial domains and spatially variable genes by graph convolutional network https://www.nature.com/articles/s41592-021-01255-8 | |
| Student presentation | ||||
| 11 | 11/23 | Lily Wang | Topic-12: Neuroscience goes viral https://www.nature.com/articles/s41592-021-01373-3 | |
| Student presentation | ||||
| 12 | 11/30 | Wan-Chen Hsieh | Topic-13:T cell development in a dish Nature Methods volume 18, page35 (2021) https://www.nature.com/articles/s41592-020-01047-6 | |
| Student presentation | ||||
| 13 | 12/07 | Yu-Ting Lin | Topic-14: Patch-seq technique Nat Methods 19, 1340–1344 (2022) https://www.nature.com/articles/s41592-022-01662-5 | |
| Student presentation | ||||
| 14 | 12/14 | Wan-Chen Hsieh | Topic-15:Macrophages enter CAR immunotherapy Nature Methods volume 17, page 561 (2020) https://www.nature.com/articles/s41592-020-0862-4 | |
| Student presentation | ||||
| 15 | 12/21 | Pin-Chao Liao | Topic-16:Automated segmentation and tracking of mitochondria in live-cell time-lapse images Nature Methods volume 18, pages 1091–1102 (2021) https://www.nature.com/articles/s41592-021-01234-z | |
| Student presentation | ||||
| 16 | 12/28 | Pin-Chao Liao | Topic-17: Actin chromobody imaging reveals suborganellar actin dynamics Nature Methods volume 17, pages 917–921 (2020) https://www.nature.com/articles/s41592-020-0926-5 | |
| Student presentation |
成績參考
| ♠ | Course participation: 20% |
| ♠ | Presentation: 80% (40% classmates, 40% teacher) |
相關網頁
| ♠ | https://www.nature.com/nmeth/ |
| ♠ | https://www.nature.com/nmeth/articles?type=method-to-watch |
